9 Best Mattress Stain and Urine Cleaners of 2026

Kids 'N' Pets ranks first because it is the only cleaner here whose maker writes for both children and pets and names mattresses directly. The brand calls this the original Kids 'N' Pets enzyme formula, built for urine, feces and vomit, and says it breaks down the uric acid compounds that chemical cleaners leave behind. Directions are short: wipe up what you can, saturate the spot, leave it 5 minutes and wipe with a clean white cloth, or leave old stains for an hour or more. Its surface list includes mattresses, bedding, pillows, car seats and upholstery, and it also works as a laundry pre-treatment for the sheets. A 32 oz bottle was $19.99 on the brand's store when we checked, and the 128 oz size was $29.99. The formula contains fragrance and colorant; parents who want neither can look at the brand's fragrance-free Mom's Miracle at $29.99. We compared documented features and published prices and did not test it.

Rocco & Roxie is the strongest pick for pet owners. The company describes a professional strength bio-enzymatic formula whose enzymatic bacteria activate on contact with odors and stains and feed on ammonia crystals and organic matter until they are gone, and it carries the Carpet and Rug Institute Seal of Approval. Its FAQ gives a mattress instruction: spray or pour on the mattress and leave it to work. For older stains the brand suggests letting the product sit overnight, or for a couple of days, so it reaches deeper. Rocco & Roxie says the formula is chlorine free, color safe and safe around pets and children, and warns against using it in a steam cleaner because heat can deactivate the enzymes. A 32 oz bottle was $23.97 as a one-time purchase on the brand's store, with 15% off on subscription. We compared documented features and published prices and did not test it.

Biokleen Bac-Out is the pick for parents who read ingredient labels. Biokleen says its live enzyme cultures break down stains and odors into tiny particles, which keeps the cleaning going after you spray, and that it removes stains, odors and urine from pet areas as well as fabrics, laundry, carpets and furniture. The page lists every ingredient, mostly plant-derived, and says the formula has no phosphates, chlorine, ammonia, artificial fragrance or dyes. A 32 oz bottle was $15.99 on Biokleen's store when we checked, and there is a foaming spray at the same price that clings better to the side of a mattress. A fragrance-free Free and Clear version exists but comes only in a 128 oz size at $39.99. The page does not name mattresses, so test on a hidden spot first. We compared documented features and published prices and did not test it.

Skout's Honor takes a different route from the enzyme cleaners above. The company says its plant-based formula penetrates deep into carpet, upholstery and any water-safe surface to remove urine odor at the source, with no enzymes, no masking scent and no pretreatment. It is written for dog urine, from potty training a puppy to an incontinent senior dog, and a separate cat version is sold. The 35 oz trigger bottle was $15.49 on the brand's store when we checked, the lowest verified price on this list and the largest standard bottle, with 5% off on subscription. Skout's Honor is a B Corp, makes its products in the USA and backs them with a satisfaction guarantee. The page does not mention mattresses by name, so treat it as a water-safe fabric cleaner and test a hidden area first. We compared documented features and published prices and did not test it.

Puracy's Pet Stain & Odor Remover is a plant-powered spray that the company says now uses a no rinse formula for easier, faster treatments. It carries USDA Biobased and PETA Cruelty-Free certifications and lists a mannanase enzyme among its key ingredients. The directions split by age of the accident: for new ones, blot, spray, work it in and extract with water after 15 minutes; for old ones, find the spot (a blacklight helps), dilute 1:1, soak it, cover it with a weighted bag and extract after 8 hours. That routine fits a household with a handheld spot cleaner better than one with only towels. A standard bottle was on sale for $15.74 on Puracy's store when we checked, against a regular price of $20.99, and refills are sold. The product has a light cucumber and mint scent. We compared documented features and published prices and did not test it.

Nature's Miracle is one of the best known pet stain brands, and its Urine Destroyer is the line built for urine. The brand describes a powerful enzymatic formula that is tough on dog urine and the yellow, sticky residue it leaves, and says the enzymes keep working as long as any bio-based mess is still present. It has a light fresh scent and, used and stored as directed, can be used around pets. There are separate dog and cat versions plus a Plus formula. Nature's Miracle does not sell direct, so we could not confirm a US price on the maker's site and list it as pending. Its product page does not mention mattresses, so check colorfastness on the mattress cover before soaking it. We compared documented features and published prices and did not test it.

Simple Solution's Pet Urine Destroyer pairs enzymes with what the company calls 2X Pro-Bacteria, a dog-specific formula meant to break down, neutralize and permanently remove urine stains and odor and discourage future marking of the same spot. The maker says it is safe on carpet, upholstery, bedding, fabric toys and other water-safe surfaces when used as directed, and it is certified by the Carpet and Rug Institute. Directions are to saturate the area, let it sit 10 minutes, blot, and let it dry naturally, which Simple Solution says may take 24 to 48 hours; it guarantees the stain and odor are gone once dry. That long drying time is the main drawback on a mattress you need to sleep on. The brand does not sell direct, so the US price is pending. We compared documented features and published prices and did not test it.

FOLEX is on this list as a stain lifter, not an odor remover. The company describes a water-based, non-ionic surfactant that removes pet accidents, grease, ink, red wine, coffee, blood, food and most old stains from colorfast carpet, upholstery and other materials that can be safely dampened with water. There is no rinsing, vacuuming or waiting: apply a generous amount, agitate gently and blot with a clean cloth, then let the material dry. FOLEX says it is safe around children and pets when used as directed, odor-free and free of solvents, VOCs, CFCs and petroleum. Because it is not enzymatic, it will not break down the uric acid that causes lingering urine smell, so use it after an enzyme cleaner to remove any mark that remains. FOLEX sells through retailers, so we list the price as pending. We compared documented features and published prices and did not test it.

Zero Odor is the pick for noses that react to scented cleaners. The company says its patented formula reaches into fabrics to lift organic pet stains while removing the source of the odor, and that it does not mask or cover odors with fragrances or perfumes. It lists urine, feces, vomit and blood as targets and describes the product as non-toxic, non-allergenic, cruelty-free and biodegradable. The directions fit a mattress well: flush with water and blot, spray (do not pour), rub gently with a sponge, then blot again with a white paper towel, repeating for old stains. The ingredient list does include a tracer fragrance. Zero Odor's store showed prices only in Polish zloty from our location, so the US price is pending. We compared documented features and published prices and did not test it.